Sphingomyelin Assay Kit - 96 Well

Cayman`s sphingomyelin (SM) assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue color with an optimal absorption at 595 nm.Application/Colorimetric Assay, Chemical Class/Assay Kits, Chemical Class/Ceramides/Sphingolipids, Chemical Class/Lipids, Chemical Class/Small Molecule Detection (Non-EIA)Synonyms :SM Assay Kit