SET8 Methyltransferase Inhibitor Screening Assay Kit - 96 Well

SET Domain-containing Protein 8 (SET8) is a methyltransferase that selectively mono-methylates histone H4 at lysine residue 20 (H4K20), an event proven to have an important role in chromatin structure and transcriptional activation. Cayman`s SET8 Methyltransferase Inhibitor Screening Assay provides a convenient method for screening human SET8 inhibitors. The transfer of the methyl group from SAM by SET8 (provided in the kit) to the acceptor peptide (H4K20) generates S-adenosylhomocysteine, which is rapidly converted to urate and hydrogen peroxide (H2O2) using an enzyme mixture provided in the kit. The H2O2 formed is quantified using 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to produce the highly fluorescent compound resorufin (excitation 530-540 nm; emission 585-595 nm).Application/Inhibitor Screening Assay, Chemical Class/Assay Kits, Chemical Class/Methyltransferase, Research Area/Cancer, Research Area/Drug Discovery, Research Area/EpigeneticsSynonyms :SET domain-containing (lysine methyltransferase) 8; PR-Set7; SETD8