Testosterone (saliva) LUM - 96 Determinations

The principle of the following chemiluminescence immunoassay (LUM) test follows a two-step competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and a biotin-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. After washing the streptavidin-horseradish peroxidase conjugate is incubated and bound to any bound biotinylated testosterone. The washing and decanting procedures remove unbound materials. After the second washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of testosterone in the sample. A set of calibrators are used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read. Application: Method:LUM Species:Human Sample Type:Human saliva. SStorage:2-8°C Stability: Sensitivity:1.0 pg/mL. Time:1 hour incubation (RT) + 30 min. (RT) + 15 min. (R