Rat beta-2 Microglobulin ELISA Kit 1 Kit (96 Wells) - 1 Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1.  In this assay the Beta 2-Microglobulin present in samples reacts with the antiBeta 2-Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells.  After the removal of unbound proteins by washing, anti-B2M antibodies conjugated with horseradish peroxidase (HRP), are added.  These enzyme-labeled  antibodies form complexes with the previously bound B2M.  Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB).  The quantity of bound enzyme varies directly with the concentration of B2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of B2M in the test sample.  The quantity of B2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Application: Method:ELISA Species: Storage: